• Congratulations to Xuzichao Li and Lingling Zhang! Our paper was published in The EMBO Journal!

    The type I-F CRISPR variant utilizes a fused HNH nuclease domain to perform substrate cleavage instead of the signature Cas3 nuclease. Our work not only delineates the molecular details for target recognition and activation of the type I-F Cas8-HNH system, but also guides further developments leveraging this system for precise DNA editing.

    넶20 2024-09-10

  • Congratulations to Fumeng Liao and Zhikun Liu! Our paper was published in Nucleic Acids Research!

    The identified SIR2 NADase and HerA ATPase/helicase participate in antiviral process through NAD+ degradation, ATP hydrolysis, and DNA cleavage. Our study not only provides a structural basis for the functional communications between SIR2 and HerA proteins, but also unravels a novel concerted antiviral mechanism of SIR2–HerA system.

    넶11 2024-09-05

  • Congratulations to Xuzichao Li, Qiuqiu He, Xiaoshen Wang and Tongyao Wang! Our paper was published in Nature!

    The newly identified type VII CRISPR–Cas candidate system uses a CRISPR RNA-guided ribonucleoprotein complex formed by Cas5 and Cas7 proteins to target RNA. Our study elucidates critical molecular details underlying the assembly of the interference complex and substrate cleavage in the type VII CRISPR–Cas system, which may help rational engineering of the type VII CRISPR–Cas system for biotechnological applications.

    넶31 2024-08-29

  • Congratulations to Xuzichao Li and Xiaoshen Wang! Our paper was published in Nature Communications!

    The Silent Information Regulator 2 (SIR2) protein is implicated in antiviral response by depleting the cellular metabolite NAD+. The defense-associated sirtuin 2 (DSR2) effector, a SIR2 domain-containing protein, protects bacteria from phage infection by depleting NAD+, while an anti-DSR2 protein (DSAD1) is employed by some phages to evade this host defense. Our results provide molecular insights into the regulatory mechanism of SIR2-dependent NAD+ depletion in antiviral immunity.

    넶13 2024-08-29

  • Congratulations to Yongjian Ma and Xuzichao Li! Our paper was published in PLOS Biology!

    Here, we systematically screened Variovorax iad operon products and identified 2 proteins, IadK2 and IadD, that directly associate with auxin indole-3-acetic acid (IAA), identified the key components and underlying mechanisms involved in IAA transformation by Variovorax and brings new insights into the bacterial turnover of plant hormones, which would provide the basis for potential applications in rhizosphere optimization and ecological agriculture.

    넶151 2023-08-08

  • Congratulations to Xiaoshen Wang, Xuzichao Li and Lingling Zhang! Our paper was published in Cell Research!

    Short prokaryotic Argonaute proteins are associated with NADase domain-containing proteins (TIR-APAZ or SIR2-APAZ). They confer immunity against mobile genetic elements, by inducing NAD+ depletion upon recognition of target nucleic acids. Here, we report multiple cryo-EM structures of NADase/Ago complexes from two distinct systems (TIR-APAZ/Ago and SIR2-APAZ/Ago) and reveal the mechanistic diversity and similarity of Ago protein-associated NADase systems in prokaryotic immune response.

    넶118 2023-08-08

  • Congratulations to Qiuqiu He! Our paper was published in Nature Communications!

    The multifunctional GSDMB protein is an important molecule in human immunity. The pyroptotic and bactericidal activity of GSDMB is a host response to infection by the bacterial pathogen Shigella flexneri, which employs the virulence effector IpaH7.8 to ubiquitinate and target GSDMB for proteasome-dependent degradation. Furthermore, IpaH7.8 selectively targets human but not mouse GSDMD, suggesting a non-canonical mechanism of substrate selection.

    넶139 2023-01-13

  • Congratulations to Xiaoshen Wang, and Yanan Wen! Our paper was published in Nucleic Acids Research!

    The type III-E CRISPR–Cas systems are newly identified adaptive immune systems in prokaryotes that use a single Cas7–11 protein to specifically cleave target RNA. Cas7–11 could associate with Csx29, a putative caspase-like protein encoded by the gene frequently found in the type III-E loci, suggesting a functional linkage between the RNase and protease activities in type III-E systems.

    넶72 2022-12-09

  • Congratulations to Xiaoshen Wang and Yi Zhang! Our paper was published in Nature Microbiology!

    Recently discovered type III-E CRISPR-Cas systems use the single Cas7-11 effector to cleave foreign RNA. Here the authors use cryo-electron microscopy and biochemical approache s to reveal the mechanisms underlying crRNA maturation, target RNA recognition and cleavage, and communica ation with the protease Csx29 by Cas7-11.

    넶83 2022-10-27

  • Congratulation to Xiao Liang! Our paper was published in Nature Chemical Biology!

    The E3 ligase TRIM7 has emerged as a critical player in viral infection and pathogenesis.We identify norovirus and SARS-CoV-2 proteins, and physiological proteins, as new TRIM7 substrates.TRIM7 could trigger the ubiquitination and degradation of these substrates, possibly representing a new Gln/C-degron pathway. These findings unveil a common recognition mode by TRIM7, providing the foundation for further mechanistic characterization of antiviral and cellular functions of TRIM7.

    넶107 2022-10-19

  • Congratulations to Xiaoshen Wang and Xuzichao Li! Our paper was published in Nucleic Acids Research!

    AcrIIA17 inhibits Cas9 activity through interference with Cas9-sgRNA binary complex formation. In contrast, AcrIIA18 induces the truncation of sgRNA in a Cas9-dependent manner, generating a shortened sgRNA incapable of triggering Cas9 activity.

    넶75 2022-10-19
넳 넲